Evaluating the Antibacterial Activity of Prepared Chitosan Nanoparticles Loaded with Grape Seed Extract Incorporated into Dental Adhesive

Authors

1 Assistant Lecturer of Dental Biomaterials, Faculty of Oral and Dental Medicine, Misr International University,Cairo,Egypt

2 Professor of Dental Biomaterials, Dean of Faculty of Dental Medicine for Girls Al-Azhar University,Cairo,Egypt

3 Assistant Professor of Dental Biomaterials, Faculty of Dental Medicine for Girls Al-Azhar University,Cairo,Egypt

Abstract

Purpose: To evaluate the effect of prepared chitosan nanoparticles loaded with grape seed extract on the antibacterial efficiency against Streptococcus mutans of dental adhesive impregnated with these nanoparticles. Materials and methods: Chitosan nanoparticles (CsNps) were prepared and loaded with grape seed extract (GSE) by ionic gelation method with ratio 2:1, then freeze-dried to obtain CsNps loaded with GSE powder. Characterization of prepared nanoparticles was done using Transmission electron microscope (TEM) and Fourier transmission infrared spectroscopy (FTIR). The CsNps loaded with GSE powder was added to dental adhesive and the antibacterial activity was evaluated via direct contact test. Data were collected, tested for normality and subjected to repeated measures ANOVA followed by post-Hoc tests for pair-wise multiple comparisons. Results: TEM and FTIR ensured the encapsulation between GSE and CsNps. The particle size of CsNps with GSE ranged from 135 to 239nm. After adhesive modification with prepared nanoparticles, the antibacterial evaluation showed significant increase in bacterial inhibition (P < 0.001). Conclusion: The incorporation of CsNps loaded with GSE is a promising approach to enhance the antibacterial activity of the tested dental adhesive.

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