Evaluation of the Effect of Different Concentrations of Rosehip on Gingival Fibroblastic Cell Culture

Authors

1 Dentist at Egyptian Ministry of Health.

2 Professor and Head of Oral Medicine, Periodontology, Oral Diagnosis and Oral Radiology Department Faculty of Dental Medicine for Girls Al-Azhar University, Cairo, Egypt.

3 Professor of Applied organic Chemistry, chemistry Department, Faculty of Science for Girls Al-Azhar University, Cairo, Egypt

4 Lecturer of Oral Medicine, Periodontology, Oral Diagnosis and Radiology, Faculty of Dental Medicine for Girls Al-Azhar University, Cairo, Egypt.

Abstract

Purpose: The purpose of this study was to see how varied rosehip concentrations affected gingival fibroblastic cell culture. Materials and Methods: we prepared different concentrations of Rosehip oil (RHO) and RHO with Solvent (RHO/S). Gingival fibroblast cells were isolated from gingivitis patient. Gingival fibroblast cells were cultured, characterized, and analyzed in two subgroups. 1st subgroup: gingival fibroblast cells were treated with RHO RHO\S at different concentrations (5, 10) µg/mL, counted using a hemocytometer. Trypan blue and the cell proliferation assay3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) were done at 24, 48, 72hours. The half-maximal effective concentration (EC50) of RHO and RHO/S was calculated, and apoptosis was detected after 72 hours of treatment with RHO and RHO/S (EC50). 2nd subgroup: untreated cells. Results: A statically significant (P≤0.05) positive correlation was observed between different concentrations of RHO, RHO\S, cell count, cell proliferation and viability. Conclusion: RHO and RHO/S can be effectively used on gingival fibroblast cells; no in vitro cytotoxicity was detected for RHO and RHO/S.

Keywords

Files

Share

How to cite

Statistics