Evaluation of The Effect of Surface Modification of 3D-Printed Poly L-lactic Acid Scaffolds by Freeze-Dried Platelet-Rich Fibrin on Migration and Attachment of Dental Pulp Stem Cells

Authors

1 Research assistant at Oro dental genetics, Human genetics and genome research institute, National Research Centre, Giza

2 Dean of Faculty of Dental Medicine for Girls, Professor of Dental Biomaterials, Al-Azhar University, Cairo, Egypt

3 Professor of Oro-Dental Genetics, Human Genetics and Genome Research Institute, National Research Centre, Giza, Egypt

4 Assistant Professor of Oro-Dental Genetics, Human Genetics and Genome Research Institute, National Research Centre, Giza, Egypt

5 Instructor of Oro Dental Genetics, Human Genetics and Genome Research Institute, National Research Centre, Giza, Egypt.

Abstract

Purpose: The goal of that research is to appraise the surface modification effect of 3D-printed poly L-lactic acid (PLLA) by freeze-dried platelet-rich fibrin on some of the properties of stem cells from dental pulp. Materials and methods: Stem cells extracted from dental pulp (DPSCs) were isolated and cultured from teeth indicated for extraction from donors with ages ranging from 19 to 25. Seeding of the 6th passage of the isolated cells was done on 3D printed PLLA scaffolds sterilized by (Gama) radiation. The Scaffold surface was modified using platelet-rich fibrin (PRF) by a freeze-thaw cycle. Scaffolds before and after modification were examined for characterization under a scanning electron microscope. Assessment of the characteristics of seeded dental pulp stem cells represented in cell attachment and migration on modified and unmodified scaffolds was done. Cell attachment was assessed using an electron microscope while evaluation of cell migration was done using a transwell migration assay. Results: Scaffolds modified by surface coating using freeze-dried PRF provided a better attachment substrate for stem cells extracted from dental pulp (DPSCs). The migration ability of DPSCs was significantly increased toward modified scaffolds. Conclusion: Modifying PLLA 3D-printed scaffolds’ surface with freeze-dried platelet rich with fibrin could serve as a better scaffold choice for several applications of tissue engineering.

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