Effect of Tideglusib Drug as Capping Material by Inducing Stem Cell Differentiation in Rabbit Teeth

Authors

1 Assistant Lecturer of Oral and Dental Biology Department, Faculty of Oral and Dental Medicine, Sinai University, Al-Arish, Egypt

2 Professor of Removable Prosthodontics, Head of Removable Prosthodontics Department, Former Dean of the Faculty of Dental Medicine for Girls, Al-Azhar University, Cairo, Egypt

3 Professor and Head of Oral and Dental Biology Department, Faculty of Dental Medicine for Girls, Al-Azhar University, Cairo, Egypt

Abstract

Purpose: This study was conducted to evaluate the effect of GSK-3 inhibitors Tideglusib on stem cell stimulation for tooth repair. Subjects and methods: Twenty- five adult male rabbits were divided into three main groups: Control group I had 5 molars without pulp exposure, MTA Group II had 10 molars with pulp exposure capped with MTA subdivided into Group IIa capped for 4 weeks and Group IIb capped for 6 weeks and Tideglusib Group III had 10 molars with pulp exposure capped by 50 nM Tideglusib dissolved and diluted in Dimethyl sulfoxide carried by kolspon sponge. Group III was subdivided into Group IIIa which was capped for 4 weeks and Group IIIb which was capped for 6 weeks and sealed by Resin Modified Glass Ionomer. Part of the samples was decalcified for 6 weeks in 19% EDTA for histological examination with Hematoxylin and Eosin stain and immunohistochemical stains. The other part was processed for transmission electron microscopic examination. Results: The histological results showed a distinct dentin bridge in Tideglusib Group IIIb than the other groups, while the immunohistochemical stain with OCTA-4 revealed high intensity at Group IIIb. Lastly, ultrastructural results showed high activity of odontoblatic cells and angiogenesis in Tideglusib Group IIIb with preservation of vitality. Conclusion: Tideglusib drug can form reparative dentin via stem cell stimulation.

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