Evaluation of Antimicrobial Efficacy of Propolis and Chamomile as Root Canal Irrigant on Enterococcus Faecalis

Document Type : Original Article

Authors

1 Demonstrator at pedodontics department, Faculty of Oral and Dental Medicine, Badr University in Cairo, Egypt

2 Associate professor and Head of Pedodontics and Oral Health Department, Faculty of Dental Medicine for Girls, Al-Azhar University, Cairo, Egypt

3 Lecturer of Pedodontics and Oral Health, Faculty of Dental Medicine for Girls, Al-Azhar University, Cairo, Egypt

Abstract

Purpose: To evaluate the antibacterial effectiveness of Propolis and Chamomile as root canal irrigants on Enterococcus faecalis for Primary Teeth. Materials and methods: 45 extracted primary anterior teeth were selected. Access cavity and mechanical preparation of root canals were done; teeth were sterilized in autoclave at 121° for 20 minutes. These teeth were inoculated with Enterococcus faecalis with reference strain ATCC 19433.Then, grouped into three groups which were irrigated with 2% chlorohexidine, Propolis and German chamomile respectively. Samples were cultured on bile auscline agar then incubated at 37°C for 24 hours. Counting of colony forming units before and after irrigation was performed. Results: Chamomile had a higher antimicrobial effect than Propolis and both showed an antimicrobial effect lower than chlorohexidine. ANOVA test and Bonferroni post hoc test revealed that chlorohexidine recorded a barely significantly greater percentage of bacterial reduction in comparison to other 2 groups (p=0.05). The percentage reduction in Propolis and Chamomile groups was not significantly different. Conclusion: Propolis and chamomile can be utilized as a natural alternative for chlorhexidine in root canal irrigation, even if its concentration might need to be higher or be applied for an extended amount of time inside the canals.

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